프로토콜
얼마나 길게, 몇 번, 얼마나 자주: 시행과 간격
하루 두 번의 시행이 네 번 못지않을 수 있습니다. 노트가 말하는 다른 내용도 소개합니다.
시행은 얼마나 길어야 할까요? 하루에 몇 번? 사이 간격은? 원래 노트는 실용적인 답을 주며, 랫과 마우스 사이에 조금 차이가 있습니다. 각각 원래의 말 그대로(영어) 나란히 싣습니다.
랫의 경우
원래 학습 노트는 랫이 약 2분이 지나도 플랫폼을 찾지 못하면 건져 내고, 플랫폼 위에 약 15초 동안 두어 주위를 둘러보게 하라고 제안합니다. 프로브 시행은 1~2분 동안 진행합니다. 전문은 플랫폼 찾기 학습과 랫은 꾀를 부릴 수 있다에서 읽어 보세요.
마우스의 경우
수영 시간과 간격
1. We use 2 mins in a 2m pool with a 15 cm platform. If your pool is as small as 1m, I would swim them for 1 min, & when they fail to find the platform, guide them to it so they climb on. Ideally you leave them there for 10 seconds, so they assess the surroundings. In practice they will make a leap for it if you let them. So as they climb on, step well-back & try to ensure they stay on for 10 seconds. If they immediately take to the water & keep swimming, intervene again to guide them back. They have to learn that rescue is dependent on staying on that platform.
2. If they leap for you before you retreat, you may as well catch them, as at least they have got the idea. You should find that on the first day your mice mostly fail to find the platform. Then on subsequent dates their efforts are greeted with increasing success. If you leave them too long at the beginning, swimming around without success, they will associate the swim with failure & panic.
3. We find that mice given 2 trials per day learn nearly as quickly as 4 times a day. If you are worried about the stamina of elderly mice, cut back to 2 per day. Our mice look forward to their swim (they rattle the tops of their cages when my postdoc comes to collect them). We suspect they possibly enjoy it too much, and extend the swim-time because the alternative is to go back in a boring cage. Then we tried lowering the temperature of the pool by 2 deg, and they went on strike ! What I was leading-up to is that if the mice are not panicking, I suspect the swim is not traumatic, and they enjoy doing laps of the pool.
4. Intervals between swims is important. We use 30 mins, as being a good way to reinforce learning.
원문(영어): Dr Roger J Morris, 1998, Richard Baker의 편집자 주 포함. Copyright (C) 1998 RJ Morris & HVS Image. 변경 없이 그대로 게재. Copyright Richard Baker© 2013. All Rights Reserved.
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