Measures

Quadrant time and quadrant entries

The classic probe-trial measure: did it search in the right place?

In a probe trial, an animal that remembers where the platform was spends more time in that quadrant. Dr Richard Anderson's 1998 notes describe comparing "the time the mouse spends swimming in the former platform quadrant with the time in the other three".

Why it matters

More time in the quadrant where the platform was shows the subject remembers its location.

More detail

In probe trials, a significant preference for the target quadrant, with the platform in the centre of the quadrant, shows spatial memory regardless of start point. It is useful only when platforms sit at quadrant centres; otherwise use close encounters and Gallagher proximity.

Subjects with strong spatial memory may quickly realise the platform is not there and search elsewhere, so a time slice can reveal an early preference hidden in whole-trial values (Blokland et al., 2004, found quadrant measures may overestimate spatial ability). Maei et al. (2009) found mean proximity outperformed quadrant occupancy in detecting group differences.

Why it matters

The order in which quadrants are visited can reveal the search strategy the subject used.

More detail

Entry counts show how broadly the subject explored, which time in quadrant cannot: a single long visit and many short ones look the same in cumulative time. Sequential visits suggest chaining, repetitive switches suggest perseveration, and an early direct entry suggests initial spatial memory.

Ordered entries (including doubling back) can be saved as .qad text files for sequence analysis, useful in models of rigidity, perseveration or procedural strategy use.

Why it matters

Time slices let you look at just part of a trial, such as the first seconds of a probe, when memory shows most clearly.

More detail

Set a slice start and duration in ‘Advanced’ to re-run plots and measures on that window, including in batch. The first 10–20 seconds after release are the best indicator of where the animal expects the platform to be.

Blokland et al. (2004) analysed a 2 min probe in four 30-s intervals: target quadrant preference was present in each, but annulus time changed across intervals, and they concluded that 60-s probes may underestimate spatial ability. Slices do not give heading angle or close-encounter measures, and Direct Path classifications are not produced for slices.

Time in target quadrant (QUAD1%sc etc.)

In probe trials, a preference for the target quadrant regardless of start point indicates spatial memory, but only when platforms sit at quadrant centres. Otherwise use close passes and Gallagher measures.

More detail

Quadrants start at NE and go clockwise (on screen the layout is 4 1 / 3 2), and results include the platform number used. Whole-trial quadrant time can mislead, so use average speed as a control and time slices.

Quadrant time is also useful during learning to see strategy develop, and in reversal to detect perseveration in the old quadrant. The software's quadrants are fixed, so N/S/E/W-centred quadrants need a custom module or a change of set-up.

Quadrant entries and sequence

The quadrant entry sequence lists the order in which the subject visited the quadrants. It helps you see the strategy behind the numbers.

More detail

Sequential visits round the quadrants suggest chaining; repeated switching suggests perseveration; an early direct entry suggests initial memory; a random order suggests no strategy.

With the quads-list box checked, ordered entries (including doubling back) are saved as .qad text files. Quadrants are NE, SE, SW, NW with North at the top of the image.

Time slices

A time slice analyses only part of a trial, such as the first 30 s of a probe. Slice start 0 and Slice time 30 give the first 30 s; start 30 and time 30 give the next 30 s.

More detail

Slice latency is the time to reach the platform within that slice, or the slice duration if the platform is not reached in it. Name each results file after its slice. Probe analyses often use the first 10–20 s.

Slices do not give heading angle or close encounter measures; latency and path are shorter and target quadrant % may be larger. Slice plots omit start, heading and end markers.

Questions people ask

When is percentage time in the target quadrant a good measure?

In probe trials, a preference for the target quadrant regardless of start point indicates spatial memory, but only when platforms sit at quadrant centres. Otherwise use close passes and Gallagher measures. Time slices reveal early preference.

More detail

Percentage time in the target quadrant is the share of the trial spent in the quarter of the pool that contains (or contained) the platform; with four quadrants, chance is 25%. It is a good measure in probe trials when platforms sit at quadrant centres. It is also useful during learning to see strategy develop, and in reversal to detect perseveration in the old quadrant. Whole-trial quadrant time can mislead, so use average speed as a control and time slices.

Going deeper

Quadrant time is coarse: a subject circling at the platform's distance from the centre can score well above chance without knowing the exact location, and a subject that finds the location early and then extinguishes dilutes the score. Close passes, Gallagher proximity and a first-slice analysis are more precise.

Related: What do quadrant entries and their sequence tell me? · How are quadrants numbered, and can I use N, E, S, W quadrants?

What do quadrant entries and their sequence tell me?

Quadrant entries are the number of times the subject crosses into each quarter of the pool, and the sequence is the order of those entries. Many entries can indicate hyperactivity, few apathy; repeated entries to one or two quadrants suggest perseveration; trained subjects make fewer entries concentrated on the target.

More detail

A quadrant is one of the four equal quarters into which the pool is divided, numbered clockwise from north-east, and quadrant entries count how often the subject crosses into each one. Many entries can indicate hyperactivity and few apathy; trained subjects make fewer entries, concentrated on the target quadrant. Sequential visits round the quadrants suggest chaining; repeated switching suggests perseveration; an early direct entry suggests initial memory; a random order suggests no strategy. With the quads-list box checked, ordered entries (including doubling back) are saved as .qad text files.

Going deeper

Quadrants are NE, SE, SW and NW with North at the top of the image. Entry counts rise with speed and path length, so compare them with average speed, and use the sequence rather than the count when judging strategy.

Related: When is percentage time in the target quadrant a good measure? · How are quadrants numbered, and can I use N, E, S, W quadrants?

How are quadrants numbered, and can I use N, E, S, W quadrants?

Quadrants start at NE and go clockwise: 1 = NE, 2 = SE, 3 = SW, 4 = NW (North = top of the image). Quadrants centred on N, E, S, W need a custom module; alternatively rotate the camera 45° or reposition platforms so targets sit in the standard quadrants.

More detail

On screen the layout is 4 1 / 3 2. Results include the platform number used. The software's quadrants are fixed; N/S/E/W-centred quadrants need a custom module, or you can rotate the camera 45° (rotating the set-up on screen) or move platform positions and cues to the standard quadrant centres. Quadrant measures are only meaningful when platforms are at quadrant centres; otherwise use close passes and Gallagher measures.

Going deeper

Ordered quadrant entries (Quad Entries, with the start quadrant omitted) can be saved as separate files.

Related: When is percentage time in the target quadrant a good measure? · How do I get pool circling to tell circling from other search patterns?

On hvsimage.com: preference for the target quadrant and quadrant entries.

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